{"id":3405,"date":"2019-12-04T18:22:34","date_gmt":"2019-12-04T18:22:34","guid":{"rendered":"http:\/\/apmaldi.com\/main\/?p=3405"},"modified":"2019-12-04T18:24:39","modified_gmt":"2019-12-04T18:24:39","slug":"new-subapmalding-publication-available","status":"publish","type":"post","link":"https:\/\/www.apmaldi.com\/main\/new-subapmalding-publication-available\/","title":{"rendered":"New SubAPMALDI(ng) Publication Available"},"content":{"rendered":"\n<p>New paper related to our SubAPMALDI(ng) ion source has been just published in the Analytical Chemistry journal. <\/p>\n\n\n\n<p><a href=\"https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acs.analchem.9b03909\" target=\"_blank\" rel=\"noreferrer noopener\" aria-label=\"High Spatial Resolution Imaging Mass Spectrometry Reveals Chemical Heterogeneity Across Bacterial Microcolonies (opens in a new tab)\">High Spatial Resolution Imaging Mass Spectrometry Reveals Chemical Heterogeneity Across Bacterial Microcolonies<\/a><\/p>\n\n\n\n<p>Rita de Cassia PessottiBridget L. HansenVineetha M. ZachariaDaniel PolyakovMatthew F. Traxler*<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Abstract<\/h2>\n\n\n\n<p> Microbes interact with the world around them at the chemical level. However, directly examining the chemical exchange between microbes and microbes and their environment, at ecological scales, i.e., the scale of a single bacterial cell or small groups of cells, remains a key challenge. Here we address this obstacle by presenting a methodology that enables matrix-assisted laser desorption\/ionization (MALDI) imaging mass spectrometry (IMS) of bacterial microcolonies. By combining optimized sample preparation with subatmospheric pressure MALDI, we demonstrate that chemical output from groups of as few as \u223c50 cells can be visualized with MALDI-IMS. Application of this methodology to\u00a0<em>Bacillus subtilis<\/em>\u00a0and\u00a0<em>Streptomyces coelicolor<\/em>\u00a0revealed heterogeneity in chemical output across microcolonies and asymmetrical metabolite production when cells grew within physiological gradients produced by\u00a0<em>Medicago sativa<\/em>\u00a0roots. Taken together, these results indicate that MALDI-IMS can readily visualize metabolites made by very small assemblages of bacterial cells and that even these small groups of cells can differentially produce metabolites in response to local chemical gradients. <\/p>\n","protected":false},"excerpt":{"rendered":"<p><!-- wp:paragraph --><\/p>\n<p>New paper related to our SubAPMALDI(ng) ion source has been just published in the Analytical Chemistry journal. <\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><a href=\"https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acs.analchem.9b03909\" target=\"_blank\" rel=\"noreferrer noopener\" aria-label=\"High Spatial Resolution Imaging Mass Spectrometry Reveals Chemical Heterogeneity Across Bacterial Microcolonies (opens in a new tab)\">High Spatial Resolution Imaging Mass Spectrometry Reveals Chemical Heterogeneity Across Bacterial Microcolonies<\/a><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>Rita de Cassia PessottiBridget L. HansenVineetha M. ZachariaDaniel PolyakovMatthew F. Traxler*<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n","protected":false},"author":1,"featured_media":3406,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[3],"tags":[],"class_list":["post-3405","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news","post-has-thumbnail"],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.apmaldi.com\/main\/wp-content\/uploads\/2019\/12\/ac9b03909_0005.gif?fit=500%2C288&ssl=1","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/posts\/3405","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/comments?post=3405"}],"version-history":[{"count":4,"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/posts\/3405\/revisions"}],"predecessor-version":[{"id":3955,"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/posts\/3405\/revisions\/3955"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/media\/3406"}],"wp:attachment":[{"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/media?parent=3405"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/categories?post=3405"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.apmaldi.com\/main\/wp-json\/wp\/v2\/tags?post=3405"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}