Proteomics

MALDI-TOF and Ion Trap mass spectrometer systems are "tools of the trade" in proteomics. With AP/MALDI coupled to an ion trap mass spectrometer you can quickly analyze digests, generating high quality MS/MS data for protein identification - potentially reducing the need for time consuming LC/MS/MS analysis of many samples. MALDI is known for its capacity to ionize intact proteins and peptides providing for accurate mass peptide mass mapping, while traps are routinely used for sequencing when combined with database search algorithms. The AP/MALDI technique has shown sensitivity in the attomole level range for protein digest samples, high throughput automated analysis, and useful MS/MS fragmentation data.

Typical protocols for sample preparation in proteomics applications can be found here.

Typical Spectra

AP/MALDI-MS analysis of a peptide mixture: (B-Neoendorphin, FW 1100.3; Angiotensin I, FW 1296.5; Substance P, FW 1347.6; Renin Substrate Tetradecapeptide, FW 1760.0). 12.5 fmol of each peptide. Glycerol: water (1:3) matrix

Relevant References:

AP/MALDI-MS analysis of a peptide mixture: (B-Neoendorphin, FW 1100.3; Angiotensin I, FW 1296.5; Substance P, FW 1347.6; Renin Substrate Tetradecapeptide, FW 1760.0). 12.5 fmol of each peptide. Glycerol: water (1:3) matrix

Versatile Protein Identification using AP-MALDI Ion Trap Mass Spectrometry
JE Meza, D Yi , CA Miller, PD Perkins
Current Trends in Mass Spectrom. 2004; 19(5S): 14-26.

Automated protein identification using atmospheric pressure matrix-assisted laser desorption/ionization
JT Mehl, JJ Cummings, E Rohde , NN Yates
Rapid Commun. Mass Spectrom. 2003; 17: 1600-1610.

Analysis of post-translationally modified peptides and proteins by APMALDI-oaTOFMS
J Tamura, K Nagatomo, T Morita, Y Nishimura, RB Cody, P Tan
ASMS 2004; ThPN 267.

Improved sensitivity for Protein Digest Analysis with High Capacity AP-MALDI Trap
PD Perkins, BD Miller, CA Miller, A Mordehai
ASMS 2003; TPX 458.

Structure determination of MHC class I peptides of renal carcinoma cells on an AP-MALDI/ESI ion trap and a MALDI-PSD-TOF instrument
VA Thieu, D Kirsch, T Flad , CA Mueller, B. Spengler
ASMS 2003; ThPS1 329.

Fragmentation of cationized phosphotyrosine containing peptides by atmospheric pressure MALDI/ion trap mass spectrometry
SC Moyer, CE VonSeggern, RJ Cotter
J. Am. Soc. Mass Spectrom. 2003; 14: 581-592.

Atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) on a quadrupole ion trap mass spectrometer
SC Moyer , LA Marzilli, AS Woods, VV Laiko, VM Doroshenko, RJ Cotter
Int. J. Mass Spectrom. 2003; 226: 133-150.