Bacteria

AP/MALDI for Bacteria Microbial detection using AP/MALDI is a method that has gained much attention in recent times due to its application in bioagent detection.  AP/MALDI has shown the ability to detect whole cells of Bacillus species spores (for example Bacillus globigii [BG]).   The MS/MS data generated from AP/MALDI can be submitted to publicy-available protein databases, like Mascot, for search and identification purposes.

Typical Spectra:

AP/MALDI-MS of Bacillus globigii

Relevant References: Detection of cyclic lipopeptide biomarkers from Bacillus species using atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry 
AJ Madonna, KJ Voorhees, NI Taranenko, VV Laiko, VM Doroshenko  
Anal. Chem. 2003; 75: 1628-1637.

Exploring AP/MALDI/MS and AP/MALDI/FAIMS/MS as novel instrumental approaches for biological agent analysis
AC Mitchell-Roberts, RA Yost
ASMS 2004; MPA 013.

Atmospheric pressure MALDI facilitates proteomics-based analysis of Bacillus spore mixtures
PA Pribil, G Black, E Patton, VM Doroshenko, C Fenselau
ASMS 2004; MPA 002.

Rapid characterization of Bacillus spores targeting species-unique peptides produced with an atmospheric pressure matrix-assisted laser desorption/ionization source (PDF file)
Patrick A. Pribil, Elizabeth Patton, Gavin Black, Vladimir Doroshenko and Catherine Fenselau
J. Am. Soc. Mass Spectrom. 2005; 40: 464-474