Bacteria

AP/MALDI for Bacteria Microbial detection using AP/MALDI is a method that has gained much attention in recent times due to its application in bioagent detection.  AP/MALDI has shown the ability to detect whole cells of Bacillus species spores (for example Bacillus globigii [BG]).   The MS/MS data generated from AP/MALDI can be submitted to publicy-available protein databases, like Mascot, for search and identification purposes.

Typical Spectra:
AP/MALDI-MS of Bacillus globigii

Application of Atmosperic Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Rapid Identification of Neisseria Species (PDF file)
Seshu K. Gudlavalleti, Appavu K. Sundaram, Jane Razumovski, and Vladimir Doroshenko
Journal of Biomolecular techniques, Volume 19, Issue 3, July 2008

Rapid characterization of Bacillus spores targeting species-unique peptides produced with an atmospheric pressure matrix-assisted laser desorption/ionization source (PDF file)
Patrick A. Pribil, Elizabeth Patton, Gavin Black, Vladimir Doroshenko and Catherine Fenselau
J. Am. Soc. Mass Spectrom. 2005; 40: 464-474

Exploring AP/MALDI/MS and AP/MALDI/FAIMS/MS as novel instrumental approaches for biological agent analysis
AC Mitchell-Roberts, RA Yost
ASMS 2004; MPA 013.

Atmospheric pressure MALDI facilitates proteomics-based analysis of Bacillus spore mixtures
PA Pribil, G Black, E Patton, VM Doroshenko, C Fenselau
ASMS 2004; MPA 002.

Relevant References: Detection of cyclic lipopeptide biomarkers from Bacillus species using atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry 
AJ Madonna, KJ Voorhees, NI Taranenko, VV Laiko, VM Doroshenko  
Anal. Chem. 2003; 75: 1628-1637.